Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® NF-κB p65 siRNA I (Mouse Specific) (+), or SignalSilence ® NF-κB p65 siRNA II (Mouse Specific) #6339 (+), using NF-κB p65 (D14E12) XP ® Rabbit mAb #8242 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The NF-κB p65 (D14E12) XP ® Rabbit mAb confirms silencing of NF-κB p65 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.